Blood Management
Chapter 23 Blood Management E. Michael Keating and Trevor R. Pickering Key Points • Preoperative evaluation and treatment of anemia are essential. • Preoperative hemoglobin of less than 13 g/dL indicates a high…
Mortality After Total Hip Arthroplasty
Chapter 25 Mortality After Total Hip Arthroplasty Wadih Y. Matar, Armin Aalami Harandi and Javad Parvizi Key Points • Most deaths are related to cardiorespiratory complications. • Reported mortality rates at 30…
Minimally Invasive Hip Arthroplasty
Chapter 22 Minimally Invasive Hip Arthroplasty R. Michael Meneghini and Mark W. Pagnano Key Points • The risks and benefits of minimally invasive surgical approaches for total hip arthroplasty (THA) remain…
Materials in Hip Surgery: Metals as a Bearing Material
Chapter 8 Materials in Hip Surgery Metals as a Bearing Material Sophie Williams and John Fisher Key Points • Metal (usually >0.2% carbon cobalt chromium molybdenum) is used as a bearing…
Pressure Myography to Study the Function and Structure of Isolated Small Arteries
Fig. 1 Schematic drawing of the pressure myograph setup used for the described protocols 1. Regular microscope. 2. Micro-forceps, micro-scissors, and petri dish for dissection. 3. Glass pipettes…
Wire Myography to Study Vascular Tone and Vascular Structure of Isolated Mouse Arteries
Fig. 1 Tools to dissect and mount the vessels into the wire myograph. The small petri dish contains 2-cm-long wires. The blue mark on the petri dish marks a length…
Flow Cytometric Analysis of Immune Cells Within Murine Aorta
Fig. 1 Schematic describing aortic flow cytometry protocol 2 Materials 2.1 Harvest Aorta 1. Microdissection tools. 2. Surgical microscope (e.g., Diagnostic Instruments Inc., Sterling Heights, Michigan). 3. 1×…
Quantification of Apoptosis in Mouse Atherosclerotic Lesions
Fig. 1 Morphology of normal and apoptotic smooth muscle cell nuclei. (a) Control mouse carotid artery demonstrating normal nuclear morphology. (b) Carotid artery showing nuclear condensation (filled arrows) compared to…
Immunostaining of Macrophages, Endothelial Cells, and Smooth Muscle Cells in the Atherosclerotic Mouse Aorta
Fig. 1 Gravity flow apparatus for vascular perfusion. Perfusant is suspended at a height of 60 cm above the mice to be perfused so as to maintain a pressure of…
Isolation and Culture of Aortic Smooth Muscle Cells and In Vitro Calcification Assay
Fig. 1 Double-collagenase digestion procedure for VSMC isolation from aorta 3.2 Mouse Aorta Dissection 1. Select mice from which aortas will be dissected. Usually 5–10 mice are required for…
