Fig. 1 Representative image of Ki-67 immunohistochemical staining in mouse early-stage atherosclerotic plaques. apoE-KO mice were fed a high-fat/high-cholesterol diet for 6 weeks. 6 μm-thick histological sections were obtained from…
Mouse number BW (g) Glucose solution volume (μl) to inject Glucose levels (mg/dL) 0 min 15 min 30 min 60 min 120 min 1 2 3 4 5 6 …
, where [Fluo] i and [Exc] i represent the fluorescence and excitation image for source position i, respectively, and s is a smoothing factor that prevents division by very small…
Fig. 1 Surgical preparation and stabilization of the mouse carotid artery. Numbers indicate the different elements and tissues involved in the procedure. (a) Mice are immobilized in decubitus position, the…
Fig. 1 Schematic drawing of the tandem stenosis. ApoE−/− mice at 6–8 weeks of age are fed with Western diet containing 22 % fat and 0.15 % cholesterol and tap…
Fig. 1 Illustrative example of bone marrow transplantation to generate mixed chimeras in mice. Six- to eight-week-old recipient mice are irradiated with myeloablative doses of radiation. In this example, femurs…
Resolving gel (%) 5 7.5 10 12 15 Total vol 8 ml 8 ml 8 ml 8 ml 8 ml ddH2O 4.5 ml 3.8 ml 3.2 ml 2.6 ml 1.8…
Fig. 1 The primary cytokines involved in atherosclerosis. In red: atherogenic cytokines. In green: atheroprotective cytokines. In blue: exact role still under investigation Fig. 2 Cytokines in the immune response…
Fig. 1 Steps in mouse aortic endothelial cell extraction and culture. (a) Dissection of the animal to expose the thoracic and abdominal cavities. (b) Isolation of the aorta and heart…
© Springer Science+Business Media New York 2015Vicente Andrés and Beatriz Dorado (eds.)Methods in Mouse AtherosclerosisMethods in Molecular Biology133910.1007/978-1-4939-2929-0_6 6. Isolation, Culture, and Polarization of Murine Bone Marrow-Derived and Peritoneal Macrophages Inés Pineda-Torra1 , Matthew Gage1, Alba de Juan2 and Oscar M. Pello1,…
