Materials in Hip Surgery: Metals as a Bearing Material
Chapter 8 Materials in Hip Surgery Metals as a Bearing Material Sophie Williams and John Fisher Key Points • Metal (usually >0.2% carbon cobalt chromium molybdenum) is used as a bearing…
MUSCULOSKELETAL MEDICINE
Pressure Myography to Study the Function and Structure of Isolated Small Arteries
Fig. 1 Schematic drawing of the pressure myograph setup used for the described protocols 1. Regular microscope. 2. Micro-forceps, micro-scissors, and petri dish for dissection. 3. Glass pipettes…
Wire Myography to Study Vascular Tone and Vascular Structure of Isolated Mouse Arteries
Fig. 1 Tools to dissect and mount the vessels into the wire myograph. The small petri dish contains 2-cm-long wires. The blue mark on the petri dish marks a length…
Flow Cytometric Analysis of Immune Cells Within Murine Aorta
Fig. 1 Schematic describing aortic flow cytometry protocol 2 Materials 2.1 Harvest Aorta 1. Microdissection tools. 2. Surgical microscope (e.g., Diagnostic Instruments Inc., Sterling Heights, Michigan). 3. 1×…
Quantification of Apoptosis in Mouse Atherosclerotic Lesions
Fig. 1 Morphology of normal and apoptotic smooth muscle cell nuclei. (a) Control mouse carotid artery demonstrating normal nuclear morphology. (b) Carotid artery showing nuclear condensation (filled arrows) compared to…
Immunostaining of Macrophages, Endothelial Cells, and Smooth Muscle Cells in the Atherosclerotic Mouse Aorta
Fig. 1 Gravity flow apparatus for vascular perfusion. Perfusant is suspended at a height of 60 cm above the mice to be perfused so as to maintain a pressure of…
Noninvasive Molecular Imaging of Mouse Atherosclerosis
Fig. 1 Treatment with minocycline and ebselen reduced endothelial permeability, plaque burden, and gadofosveset uptake. A1 through D1 and A2 through D2, Cross-sectional delayed enhancement magnetic resonance imaging (DE-MRI) fused…
Isolation and Culture of Aortic Smooth Muscle Cells and In Vitro Calcification Assay
Fig. 1 Double-collagenase digestion procedure for VSMC isolation from aorta 3.2 Mouse Aorta Dissection 1. Select mice from which aortas will be dissected. Usually 5–10 mice are required for…
In Vivo 18F-FDG-PET Imaging in Mouse Atherosclerosis
Fig. 1 PET/CT scanner and different equipment needed for anesthesia, injection of CT contrast agent, and temperature control (a) CT subsystem (see Note 3 ). (b) PET subsystem (see…
Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root
Fig. 1 Dissection tools 8. 10 ml syringes. 9. 1.5 ml Eppendorf tubes. 10. Phosphate-buffered saline (PBS): 1.54 mM KH2PO4, 155.17 mM NaCl, 2.70 mM Na2HPO4, pH…
