In Vivo 18F-FDG-PET Imaging in Mouse Atherosclerosis
Fig. 1 PET/CT scanner and different equipment needed for anesthesia, injection of CT contrast agent, and temperature control (a) CT subsystem (see Note 3 ). (b) PET subsystem (see…
Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root
Fig. 1 Dissection tools 8. 10 ml syringes. 9. 1.5 ml Eppendorf tubes. 10. Phosphate-buffered saline (PBS): 1.54 mM KH2PO4, 155.17 mM NaCl, 2.70 mM Na2HPO4, pH…
Intravital Microscopy in the Cremaster Muscle Microcirculation for Endothelial Dysfunction Studies
Fig. 1 Mouse board for cremaster muscle intravital microscopy. The board should be maintained in a constant temperature (36.5–37.5 °C) with a heating system. There are two areas in the…
Magnetic Resonance Imaging of the Atherosclerotic Mouse Aorta
Fig. 1 Representative T1-weighted MR serial images showing the abdominal aorta (white arrow) above the right renal artery of an apoE-KO mouse demonstrating large atherosclerotic lesions protruding the arterial lumen….
Quantification of In Vitro Macrophage Cholesterol Efflux and In Vivo Macrophage-Specific Reverse Cholesterol Transport
Fig. 1 Schematic diagram of the biliary macrophage-RCT pathway. Cholesteryl esters (CE) stored in lipid droplets of macrophage foam cells are hydrolyzed by neutral cholesteryl ester hydrolases. Lipid-poor nascent preβ-migrating…
In Vitro Differentiation of Naïve CD4+ T Cells: A Tool for Understanding the Development of Atherosclerosis
Fig. 1 Immunomagnetic selection of cells. (1) The biotinylated antibodies are bound to the positive cells. (2) The streptavidin-microbeads bind to the biotinylated antibodies. (3) The magnetic columns are placed…
Immunostaining of Lymphocytes in Mouse Atherosclerotic Plaque
Target antigen Host species (label) Clone Dilution Comment Mouse CD3 Hamster 500A2 1:100 Permeabilization step required Mouse CD4 Rat H129.19 1:100 Mouse CD8 Rat 53-6.7 1:100 Mouse FoxP3 Rat FJK-16s…
Detection of Intraplaque Hemorrhage in Mouse Atherosclerotic Lesions
Intervention [reference] Agea (weeks) Diet Brachiocephalic artery (%) Carotid artery (%) None [3, 4] 20 WTD 8 0 Age [5] 42–50 Chow 66–75 n.d. Collar [7] 52 WTD n.a. 8…
Generation of Aorta Transcript Atlases of Wild-Type and Apolipoprotein E-null Mice by Laser Capture Microdissection-Based mRNA Expression Microarrays
Fig. 1 Atherosclerotic arteries harbor distinct immune cell infiltrates in plaques and adventitia. The adventitia of the normal arterial wall (left) constitutively contains vasa vasorum, lymph vessels, tissue macrophages, few…
In Vitro Macrophage Phagocytosis Assay
Fig. 1 Work flow of the phagocytosis assay with estimated times for each step. MΦ, macrophages; RBCs, red blood cells; C3, complement component 3 2 Materials 2.1 Establishing Macrophage Primary…
