B Cells

14 B Cells

Key Points

Immunoglobulins (Igs) are key to B cell function by serving as both an antigen receptor and a major secreted product. The variable region binds antigen and is generated by random rearrangement of gene segments to give rise to numerous specificities. The constant region defines the isotype and mediates effector functions.

Surface Ig is the major component of the B cell receptor, which regulates B cell selection, survival, and activation. Secreted immunoglobulin mediates antigen neutralization and opsonization with uptake by phagocytic cells, complement activation, and cellular activation or inhibition through engagement of receptors for the Fc region of Ig.

B cells are generated from hematopoietic precursors in the bone marrow and undergo several stages of maturation and selection before becoming immunocompetent, naive B cells that reside in peripheral lymphoid organs. After antigen activation, B cells differentiate to memory cells and Ig-secreting plasma cells.

Follicular B cells respond to protein antigens in a T cell–dependent fashion and are the major source of B cell memory. B1 and marginal zone B cells are less dependent on T cell help, respond primarily to polysaccharide antigens, and display limited heterogeneity of the B cell receptor.

Autoreactive B cells are generated in all individuals. Multiple checkpoints extinguish autoreactive B cells during early and later stages of B cell development. One or more of these checkpoints is breached in autoimmune-prone individuals, leading to the maturation and activation of autoreactive B cells.

Overview: B Cells and Humoral Immunity

Numerous cells comprise the immune system and are required to generate innate and adaptive immune responses. Adaptive responses are characterized by immunologic memory generated during first exposure to antigen, thereby permitting a rapid response to antigen following subsequent exposure.

B cells (Figure 14-1) are lymphocytes that recognize antigens through a molecule called the B cell receptor. The B cell receptor is composed of a surface immunoglobulin molecule, which recognizes the antigen, and two associated proteins, which transduce the signal. On encounter with its antigen, a B cell begins a process of activation leading to antibody secretion and memory formation regulated by interplay with antigen-activated T cells, dendritic cells, soluble factors, and in some cases follicular dendritic cells. Both T and B lymphocytes can differentiate from naïve to memory cells, but only B cells have the capacity to fine tune their antigen receptor structure to increase its specificity and affinity, giving rise to more effective antibodies. Beyond immunoglobulin secretion, B cells regulate the immune response by cytokine secretion and antigen presentation to T cells in the context of class II molecules.

Importantly, much of the knowledge of B cell biology has been generated in mouse models. However, in this chapter, human B cell biology is described whenever possible.

Immunoglobulins: Structure and Function

The Ig molecule is critical to all aspects of B cell biology when anchored to the B cell membrane. Termed B cell receptor (BCR), it contributes to B cell maturation and survival and initiates an activation cascade following contact with antigen. At the end of the activation process, B cells can acquire the ability to secrete large amounts of Ig, intended to neutralize the antigen that elicited the response.

Structurally, Igs, also referred to as antibodies, are composed of four polypeptide chains: two identical light (L)-chains with a molecular weight of approximately 25 kDa and two identical heavy (H)-chains of 50 to 65 kDa. Each of the chains contains a folding motif that is highly conserved among proteins of the immune system, the “Ig domain.” These domains constitute the backbone of the Ig molecule and make for the interface along which the polypeptide chains pair (Figure 14-2). The quaternary structure of an immunoglobulin molecule assumes a Y-shaped conformation, which contains two functional moieties: two identical antigen-binding regions or variable regions, which are the arms of the “Y,” and a constant region, which is the base of the “Y.”1

This definition of functional moieties derives from early studies analyzing proteolytic fragments of Ig molecules. Cleavage with papain generates two identical fragments that retain antigen-binding capacity and hence are named Fab, as well as a distinct crystallizable fragment Fc that mediates immune effector functions but is unable to interact with antigen.2

The antigen-binding regions are formed by pairing of the variable domain of the L-chain (VL) to the variable domain of the H-chain (VH). In contrast to the rest of the molecule, there is a great diversity in the amino acid sequence of the variable domains, which allows for a broad repertoire of antibody molecules that can recognize a wide array of antigens. Within the variable region of the Ig molecule are discrete regions, known as complementary determining regions (CDRs) that make direct contact with antigen. The amino acid sequences of the CDR are highly variable and are flanked by more conserved amino acid sequences called framework regions. The H- and L-chain molecules each contain three CDRs and four framework regions (see Figure 14-2). The minimal antigenic determinant recognized by the H and L CDR is known as an epitope, which may be a continuous or discontinuous region on a protein, carbohydrate, lipid, or nucleic acid. The presence of two identical variable regions in a single Ig molecule confers the capacity to interact with repetitive antigenic determinants present in multivalent antigens (i.e., polysaccharides) or two separate antigen molecules containing the same antigenic determinant.1

The constant region directs the Ig effector functions that mediate the killing and removal of invading organisms and both the activation and homeostasis of the immune system. Strictly speaking, the constant region is formed by the constant domain of the L-chain (CL), which is paired to the first constant domain of the heavy chain (CH1), and the remaining constant domains of the two heavy chains (CH2 and CH3 and CH4 in IgM), paired to each other. However, most of the functions associated with the constant region are mediated by the constant domains of the H-chain.

Immunoglobulin Constant Region

The specific binding interactions that occur between the Ig variable region and antigen may be sufficient to block microbial infectivity or neutralize toxins. However, the ability to eliminate pathogens is mediated by the Fc portion of the molecule. The Fc regions of antigen-antibody complexes are made accessible to serum factors that comprise the complement cascade or to cytotoxic and phagocytic cells that mediate the destruction and removal of pathogens. In mice and humans, there are five different types of H-chain constant regions, or isotypes, designated IgM (µ), IgD (δ), IgG (γ), IgA (α), and IgE (ε)3; each is encoded by a distinct constant region gene segment present in the H-chain locus of chromosome 4 in humans or 12 in mice. Each isotype is capable of specific effector functions, and each cellular receptor for Ig initiates a distinct intracellular signaling cascade. The number of CH domains, presence of a hinge region to increase flexibility between Fab regions, serum half-life, ability to form polymers, complement activation, and Fc receptor binding vary among isotypes. Characteristics of the different Ig H-chain isotypes are presented in Table 14-1.1,3,4 These isotypes may also differ in the intracellular signaling they initiate when bound by antigen in their membrane-associated form on the B cell. It should be noted that the interplay between antibodies and the cells that bear the Fc receptors extends beyond pathogen clearance and shapes the immune response by mediating activation or inhibition of specific cell types5 and by mediating cell death.6

Immunoglobulin M

IgM is the first isotype expressed in developing B cells and the first antibody secreted during a primary immune response. It is found predominantly in serum but is also present in mucosal secretions and breast milk. Because the process that increases antibody affinity for a particular antigen (affinity maturation) has not yet been initiated during the early stages of a primary immune response, IgM antibodies usually exhibit low affinity. Their low affinity is balanced by the fact that most of the secreted IgM exists in pentameric form, generating multiple binding sites, providing high avidity for antigen and assisting the binding of large, multimeric antigens. IgM also exists as a monomer and a hexamer, but only the pentameric form is linked by the polypeptide called joining (J)-chain. The J-chain allows the active transport of IgM to mucosal secretions.7

Many of the biologic functions of IgM are mediated by its ability to activate the classic complement pathway.1 The complement cascade is composed of a series of enzymes that, on activation, mediate the removal and lysis of invading organisms. Deposition of antibody molecules or complement components on the surface of the antigen assists phagocytosis. Proteins such as antibody and complement that enhance phagocytosis are called opsonins. Once the complement cascade has been activated, monocytes, macrophages, or neutrophils engulf opsonized particles through specific receptors present on phagocytic cells such as CD21, which recognizes fragments of the C3 complement component. Activation of the complement pathway also results in the generation of the membrane attack complex, which is composed of late complement components and directly lyses C3-opsonized pathogens. Because activation of the classic complement pathway requires Fc regions to be spatially close, but also exposed, multimeric IgM is a potent activator of the classic complement pathway once it has bound its antigen. For example, hexameric IgM is between 20 and 100 times more potent as an inducer of complement activation than monomeric IgM.8

Immunoglobulin G

IgG is the most common isotype found in serum, comprising about 70% of the circulating antibody. IgG antibodies are usually of higher affinity than IgM antibodies and predominate in a secondary or memory immune response.

In humans there are four subclasses of IgG: IgG1, IgG2, IgG3, and IgG4. IgG1 and IgG3 arise in response to viral and protein antigens. IgG2 is the main antibody present in response to polysaccharide antigens, and IgG4 participates in responses to nematodes and is observed in chronic antigenic stimulation.9

All IgG subclasses exist as monomers and have a high structural similarity; however, minor differences make for distinct biologic effects. IgG3 and IgG1 are potent activators of the classic complement pathway, and IgG2 can initiate the alternative complement pathway (see Chapter 23).

All IgG subclasses engage specific Fc gamma receptors (FcγRs) present on dendritic cells, macrophages, neutrophils, and NK cells. The FcγRs on phagocytic cells, when cross-linked, mediate the removal of immune complexes from circulation and initiate antibody-dependent, cell-mediated cytotoxicity resulting in the release of granules that contain perforin, a pore-forming protein, and enzymes known as granzymes that induce programmed cell death (apoptosis) of target cells.10,11 FcγR engagement also allows the internalization and subsequent presentation of antigens in the context of MHC class II molecules.

Because IgG antibodies are the only ones that cross the placental barrier, they are critical for the survival of newborns. The transport of IgG from the maternal circulation into the fetal blood supply is mediated by the FcRn receptor.12 FcRn is also responsible for the long half-life of IgG in serum by blocking IgG catabolism.13

Immunoglobulin A

Despite its relative low concentration in serum, more IgA is produced than all other isotypes combined. Most IgA exists as secretory IgA (SIgA) in mucosal cavities and in milk and colostrum, and only a small fraction is present in serum. Two subclasses of IgA exist in humans: IgA1 and IgA2. IgA1 is mainly produced as a monomer. In contrast, polymeric IgA2 is produced along mucosal surfaces.14

Polymeric IgA exists mainly as a dimer and includes a J-chain (the same chain that links pentameric IgM). It is captured by the polymeric immunoglobulin receptor (pIgR) that is expressed on the basolateral surface of the epithelial cells and then transcytosed to the apical side. Release of IgA into mucosal secretions requires cleavage of the pIgR; a fragment known as the secretory component (SC) remains associated with secreted IgA and protects it from the action of proteases and increases its solubility in mucus, where it neutralizes toxins and inhibits the adherence of secreted IgA-coated microorganisms to the mucosal surface.15

People with IgA deficiency have reduced levels of both serum and secreted IgA and are prone to respiratory tract and diarrheal infections, as well as an increased incidence of autoimmune disorders.16 FcαR, present in the surface of neutrophils and macrophages, has been suggested to play a regulatory role in the immune system; it is not clear if the autoimmune manifestations in IgA-deficient patients are a consequence of the absence of the regulatory loop played by engagement of FcαR.17

Immunoglobulin E

IgE is involved in protection against parasitic infections but also triggers immune responses associated with allergic reactions. Only a small amount of IgE is detectable in serum, where it exists as a monomer.18 Mast cells and basophils express a high-affinity IgE Fc receptor (FcεRI) that binds free IgE. Cross-linking of the FcεR by antigen binding to the IgE induces degranulation and release of histamine, proteases, lipid mediators such as prostaglandin D2 and leukotrienes, many of which are associated with anaphylaxis.

Immunoglobulin Variable Region

The recognition of a virtually unlimited number of antigens requires a mechanism to generate Ig molecules with similarly broad specificities. The molecular basis of this process has been known for several years.21 First, the Ig molecule is composed of both heavy and light chains. These chains are encoded within distinct genetic loci residing on separate chromosomes; the H-chain locus is on human chromosome 14,22 the κ-chain locus is on chromosome 2, and the λ-chain locus is on chromosome 22.23

Within each locus, gene segments encode both the variable and constant regions of the Ig molecule. The H-chain variable region is encoded by a variable (VH), diversity (DH), and a joining (JH) segment. The L-chain is encoded by either Vκ and Jκ or Vλ and Jλ segments; it does not contain D segments. The human H-chain locus contains from 38 to 46 VH, 23 DH, and 9 JH functional genes (these numbers represent a typical haplotype, but vary among individuals). The κ-chain locus contains approximately 31 to 35 Vκ genes and 5 Jκ functional genes; the λ-chain locus contains 29 to 32 Vλ genes and 4 or 5 Jλ functional genes.24

Generation of Immunoglobulin Diversity

In a developing B cell, different VH, DH, and JH or VL and JL gene segments are randomly combined to generate a large number of different Ig molecules (Figure 14-3). This process, known as V(D)J recombination, occurs in the primary lymphoid tissue, in the absence of antigen stimulation and must be successful to continue with B cell maturation. Here, we present the molecular process, and later the functional and developmental consequences are discussed (see B Cell Development).

V(D)J recombination happens sequentially, beginning with the joining of one DH segment to one JH. Following this, a VH segment will be targeted to the rearranged DHJH fragment. The absence of an in-frame recombination leads to recombination of the second allele. Light chain recombination also occurs stepwise. First, the kappa locus is rearranged; in absence of a productive κ-chain rearrangement, the lambda locus undergoes recombination.25

The recombination machinery is composed of specific enzymes including Recombination-Activating Gene 1 (RAG-1) and the Recombination-Activating Gene 2 (RAG-2). The complex recognizes recombination signal sequences (RSS) that flank the V, D, and J gene segments. These highly conserved sequences are composed of a palindromic heptamer (7 base pairs) followed by deoxyribonucleic acid (DNA) spacers that are 12 or 23 base pairs in length and an AT-rich nonamer.26 Once the complex recognizes its target, it generates double-stranded DNA (dsDNA) breaks at the RSS sites. Next, the cellular DNA repair complex recognizes and joins the cleaved segments.

The random recombination that occurs among V, D, and J gene segments can generate a diverse Ig repertoire without the need for a large number of germline H- and L-chain genes. During H-chain recombination, nucleotides may be added at VHDH and DHJH junctions by the enzyme terminal deoxynucleotidyl transferase (TdT). These non-germline-encoded sequences are known as N additions. As long as these nucleotide changes do not disrupt the reading frame or lead to the incorporation of premature stop codons, the random addition of N sequences increases the diversity of the amino acid sequence. Further, imprecise ligation at the coding junctions may result in the loss of nucleotides, thereby also enhancing diversity.

B Cell Development

The aim of the maturation process is to generate a pool of mature B cells with a diverse repertoire of Ig specificities that can recognize foreign and pathogenic antigens without compromising the integrity of the self. Therefore the process of generation of Ig diversity is coupled with the censoring of autospecificities.

B cells, as all cells in the hemopoietic lineage, begin with the differentiation of noncommitted, undifferentiated CD34+ hematopoietic stem cells to lymphopoietic precursors with restricted lineage potential. These cells, known as common lymphoid progenitors (CLP), have the potential to give rise to NK, T, and B cells. Early B cell progenitors begin to express genes for DNA rearrangement, as well as B cell program transcription factors. Multiple transcription factors act in concert, but Ikaros, E2A, EBF, and Pax5 appear to be the most important in B cell development.27 Pax5 is considered the master transcriptional control for B cells because it is induced in early stages of B cell commitment and plays a dual role by repressing genes required for differentiation to the myelomonocytic lineage and activating B cell–specific genes such as Ig genes, CD19, and signaling molecules.28

Niches of Human B Cell Lymphopoiesis

The development of undifferentiated hematopoietic stem cells (CD34+) into mature B cells begins in the first weeks of uterine life. By the eighth gestational week, early B cell precursors can be identified in the fetal liver and omentum. From gestational week 34 and through adulthood, the bone marrow is the primary site of lymphopoiesis.29

It has been unequivocally established that there are differences between the B cells that originate during fetal and adult lymphopoiesis in mice, and it is becoming clear that these differences extrapolate to human lymphopoiesis as well. B cell precursors are susceptible to estrogen, and the maturation of maternal B cells is arrested in the pro–B cell stage during pregnancy; in contrast, fetal B cell precursors lack estrogen receptors and consequently are unaffected by exposure to hormones.30 B cells originating during prenatal life have a bias in the usage of DH and JH gene segments, and this, along with reduced expression of the enzyme TdT, leads to a more restricted repertoire with shorter CDR3s.31

Whether during fetal or adult lymphopoiesis, the maturation of B cells from pluripotential progenitors is contingent on the presence of stromal cells that provide both contact-dependent and soluble signals. Although the nature of the interactions provided by the stromal cells to create a lymphopoiesis-permissive environment is still largely unknown, they include both survival and proliferative signals. The stromal-derived factor-1 (SDF-1) is required for the homing of the early B cell precursors to sites of lymphopoiesis but also promotes differentiation.32 Interactions between VCAM-1 on the membrane of the stromal cells and its counterpart, VCAM-4, on early B cell progenitors are required for B cell differentiation. The molecules IL-7, IL-3, and the Flt3 ligand promote B cell lymphopoiesis, although IL-7 appears to be dispensable for human B cell development. Matrix molecules in the microenvironment such as heparan sulfate proteoglycan are assumed to “trap” critical soluble factors.33

B Cell Ontogeny

The stages of B cell development are defined by the state of Ig gene rearrangement and the expression of intracellular and surface proteins. The nomenclature and classification of particular stages vary slightly among different laboratories working in this field. For simplicity, we have divided the stages of B cell lymphopoiesis into early B cell progenitors, pro-B, pre-B, immature, transitional, and mature naïve cell (Table 14-2).

When a CLP begins to transcribe RNA coding for proteins required for B cell maturation, E2A and EBF, the cell becomes an early B cell progenitor. Once these two transcription factors are expressed, they enable transcription of the proteins involved in the recombination machinery (RAG1/2). The beginning of D to J recombination marks the progress to a pro-B stage.

Pro-B Cells

This stage is defined by the rearrangement of the heavy chain gene segments and synthesis of a µ-polypeptide. Pro-B cells are dependent on interactions with endothelial cells present in the stroma. The VLA-4 integrin receptor and CD44, which both mediate adhesion to stromal cells, are highly expressed at this stage and are believed to be important for continued development.34 Pro-B cells also express high levels of Bcl-2, a molecule that protects cells from apoptosis. At the onset of the pro-B cell stage, the variable gene segments of both H- and L-chain loci are in the unrearranged germline configuration but accessible to the recombination machinery. A DH gene segment on one H-chain chromosome rearranges with a JH gene segment residing on the same chromosome, often with the inclusion of nontemplate nucleotides at the junction of these two segments. Next, a VH gene rearranges to the DHJH gene fragment. Completion of VHDHJH gene rearrangement leads to the generation of an H-chain transcript that also contains the IgM constant region (Cµ), which is the constant region gene most proximal to the variable region genes on the chromosome (see Figure 14-3).

The generation of a µ-polypeptide and its subsequent expression on the surface of the cell, together with a surrogate light chain formed by the λ5 and Vpre-B polypeptides as well as the Igα/Igβ dimer, a complex known as the pre-B cell receptor (pre-BCR), marks the end of this phase of gene recombination. This constitutes a critical developmental checkpoint and the entrance to the next developmental stage known as the pre-B cell.35 The requirement for a pre-BCR complex ensures that B cells without a productive H-chain will not undergo further differentiation.

The pre-BCR transduces a signal that the VDJ rearrangement was successful and halts recombination of the second H-chain allele. This process, known as allelic exclusion, ensures that all Ig molecules generated within a single B cell are identical and have the same antigenic specificity. If no µ-chain is generated, rearrangement is initiated on the other chromosome. If the second rearrangement also results in a nonproductive H-chain molecule, the absence of pre-BCR-mediated signal induces apoptosis. The odds of generating a productive rearrangement are one in three, and consequently, approximately 50% of the cells that begin recombination will be unable to proceed along a developmental pathway.

Pre-B Cells

The pre-B cell stage is characterized by light chain recombination. Initiation of this stage requires the presence of the pre-BCR and functional signal transduction machinery.

At the onset of the pre-B cell stage, the expression of the pre-BCR induces a proliferative burst that generates daughter cells with the same heavy chain and potential for multiple specificities within daughter cells, each of which may produce a different light chain. It is unknown if expression of the pre-BCR and tonic signaling is enough to trigger these events36 or if a ligand must engage the pre-BCR.37,38 Either way, targeted disruption of genes encoding the pre-BCR complex such as the IgM transmembrane constant region domain, λ5, or the Igα and Igβ accessory molecules results in a profound decrease in developing B cells. Also, defects in the adapter molecule BLNK, λ5, or the tyrosine kinase Btk lead to a serious impairment in pre-B cell maturation.

The expression of the pre-BCR is transient. After the proliferative burst, the µ-heavy chain is present only in the cytoplasm as the pre-B cell rearranges an L-chain. The general rearrangement process is similar to V(D)J rearrangement and is dependent on Rag1/2 expression. Because TdT is not expressed in this stage, L-chains do not usually contain N sequences at the VLJL junction. At the end of this process, pairing of the newly minted light chain with the µ-heavy chain leads to the surface expression of an IgM molecule, complexed with Igα and Igβ, to form the B cell receptor (BCR). It is believed that surface expression of the BCR on immature B cells transduces signals that enforce allelic exclusion at the L-chain locus and downregulate expression of the RAG genes. This immature B cell has completed the gene rearrangement process and is now subject to repertoire selection.39

Peripheral B Cell Subsets

As B cells mature and their dependence on stromal cells decreases, they leave the bone marrow and finish their maturation in the spleen before homing to other lymphoid tissue such as the lymph nodes, tonsils, and Peyer’s patches of the intestine. It is in these secondary lymphoid organs where mature B cells interact with foreign antigen and specific humoral immune responses are activated.

Transitional B Cells

Once immature B cells egress from the bone marrow, they are called transitional cells. These cells are the earliest B cells found in the periphery in healthy subjects and move to the spleen to finish their maturation.

Transitional cells are the last B cell subpopulation that expresses the developmental marker CD24. At this stage B cells begin to express surface IgD, which harbors the same specificity as the IgM because the IgD heavy chain is encoded by the same VDJ fragments as IgM but expresses the Cδ instead of the Cµ domain. It is the expression of IgD that separates transitional cells into two different maturation stages. Transitional 1 (T1) B cells that do not express IgD are the recent bone marrow emigrants, and transitional 2 (T2) B cells that begin to express IgD represent the subsequent maturational stage.40 The existence and functional characteristics of a third transitional stage (T3) are still debated.

Transitional cells constitute a stage subject to multiple regulatory processes. First, transitional cells must compete with naïve B cells already present in the periphery for a developmental niche. Transitional B cells are extremely dependent on a B cell survival factor known as B cell–activating factor of the tumor necrosis factor family (BAFF or BLyS). In its absence, B cell development does not progress beyond the T1 stage.41 Second, T1 transitional cells are still highly susceptible to tolerance induction following BCR cross-linking. In T1 cells cross-linking of the BCR ex vivo has been shown to lead to cell death, whereas T2 cells respond to BCR cross-linking by proliferation and differentiation to the mature naïve B cell stage.

BAFF Family of Cytokines

The cytokine milieu surrounding the B cell is diverse and spatially and temporarily regulated. Although B cells are modulated by multiple cytokines, in recent years two members of the TNF family, BAFF and APRIL, have emerged as key survival factors, particularly at two regulatory points in development and differentiation: the transition from an immature to a naïve B cell in the periphery and the survival of the newly produced plasma cells. BAFF (B cell–activating factor, BLyS) and APRIL (A proliferation-inducing ligand) are proteins produced by cells that take part in the innate response such as macrophages and dendritic cells, as well as stromal cells, and are present as membrane-bound proteins or soluble trimers. They have three known receptors (BAFF-R, TACI, and BCMA) that are present on the membrane of B cells from the T2 stage to their final differentiation to plasma cells. BAFF binds the three receptors, whereas APRIL binds only TACI and BCMA. BAFF induces survival and activation of B cells when bound to BAFF-R, whereas BAFF signaling through TACI decreases the size of the B cell pool. APRIL does not participate in B cell homeostasis but seems critical to the survival of plasmablasts in the bone marrow.42

Enhanced survival and activation of autoreactive B cells have been demonstrated in mice that overexpress BAFF.43 An increase in serum levels of BAFF has been observed in some patients with lupus, rheumatoid arthritis, and Sjögren’s syndrome and is thought to contribute to pathogenesis because autoreactive B cells have a survival advantage in the presence of excess BAFF44 (Figure 14-4).

Marginal Zone B Cells

Marginal zone (MZ) B cells are a population of noncirculating mature B cells located in the marginal zone of the rodent spleen. In rodents, MZ B cells present clear phenotypic and functional differences to the cells present in the follicles, responding to blood-borne pathogens and to repetitive antigenic structures such as the ones present on polysaccharide antigens.

In the human spleen, the structural definition of the marginal zone does not correspond exactly to the area surrounding B cell follicles. However, there is a population with the functional characteristics of mouse MZ B cells: low activation thresholds, highly responsive to polysaccharide antigens and with a clear surface phenotype, can be differentiated. These cells, which are sometimes named MZ-like or unswitched memory, are not restricted to the human spleen but are found circulating in the peripheral blood, as well as in the lymph nodes, tonsils, and Peyer’s patches.46,47 They are defined as IgM+, IgD+, CD27+, CD21+, and CD1c+.47

Given that these cells possess the “memory” marker CD27, it has been suggested that they have experienced antigenic exposure; however, the presence of MZ-like B cells in subjects with X-linked agammaglobulinemia (a CD40L deficiency) suggests that even if antigen exposure has occurred, T cell help has not.

Interestingly, MZ-like B cells, although already present at birth, do not seem to be fully functional at that time; infants up to age 2 are particularly susceptible to infections by capsulated bacteria. This might be due to the functional immaturity of the cells or to the lack of development of the antigen-trapping microstructure.

Sites of B Cell Homing and Activation

After the immature B cell stage, B cells home to secondary lymphoid organs, which contain the microenvironment and architecture necessary for the retention and activation of B cells. These organs include the spleen and lymph nodes, as well as lymphoid structures in mucosal tissue (e.g., Peyer’s patches, appendix, tonsils). The secondary lymphoid tissue is adapted to trap circulating antigen and expose the B cells to it and to provide interactions with T cells and other co-stimulatory cells. Peripheral lymphoid tissue contains specialized antigen-presenting cells known as dendritic cells. In the Peyer’s patches of the intestines, foreign antigen is taken up in specialized epithelial cells known as M cells. Even though peripheral lymphoid tissues vary in structure and cellular organization, they all possess antigen-presenting cells and B cell–containing follicles surrounded by T cell–rich zones.49 As explained in the following section, antigen, T cells, and dendritic cells are required for B cell activation and differentiation into Ig-secreting plasma cells or memory B cells.

B1 cells typically home to the peritoneal and pleural cavities and, to a lesser extent, the spleen. Naïve B cells enter the peripheral circulation by passing through the endothelial lining of the sinusoids of secondary lymphoid tissue and recirculate throughout the follicles of secondary lymphoid tissues. Because naïve B cells require antigen-specific T cell help for activation, the localization of this subset near a T cell zone facilitates chance encounters between antigen-specific B cells and cognate T cells. MZ-like cells respond to antigen without cognate T cell help and in mice are well positioned to capture blood-borne antigens owing to interactions between adhesion molecules and chemokine receptors expressed in part by specialized macrophages within the marginal zone that sequester MZ B cells within the marginal sinuses.

Circulation and Homing

The entry, retention, and recirculation of B cells through secondary lymphoid organs depend on both adhesion molecules and chemokine receptors.50,51 First, expression of LFA-1 and VLA-4 is required for entry into the lymphoid tissue. Then the chemokine receptors CXCR5 and CCR7 direct localization within the tissue. The CXCR5 molecule is expressed on all mature B cells and mediates B cell migration to follicles in response to the chemokine CXCL13, which is produced by follicular stromal cells. These cells, in turn, are regulated by lymphotoxin-α made by B cells. In the follicle, the B cells scan for antigen, making contact with potential antigen-bearing cells such as follicular dendritic cells (FDC), subcapsular macrophages, and dendritic cells. If the B cell does not encounter a cognate antigen, it will exit the lymphoid organ through the efferent lymphatics in response to the molecule sphingosine-1-phosphate (S1P). Neutralization of S1P leads to sequestration of B cells in lymphoid organs.52

On antigenic encounter the B cells are retained in the lymphoid organ due to the upregulation of CCR7. The ligands for CCR7 (CCL19 and CCL21) mediate the organization of the T cell zone and attract antigen-activated B cells to this area, where cognate T cell–B cell interactions occur.

In contrast, it is assumed that MZ-like B cells respond to antigen without the help of cognate T cells. In mice, MZ B cells are present exclusively in the spleen and do not recirculate; in humans they are found in the spleen and tonsils, as well as in blood. MZ B cells localize to the outer layers of the follicles, making them among the first cells to encounter blood-borne antigens.

The interplay of chemokine expression and the induction of chemokine receptors play an important role in the germinal center response. The chemokine CXCL12 retains centroblasts in the dark zone during the process of somatic hypermutation and isotype class switching. CXCL13 regulates migration to the light zone, where survival and selection events are mediated by interactions with CXCR5-expressing follicular helper T cells and FDCs.53 Later, CXCL12 promotes the migration of plasmablasts to the bone marrow, where they undergo further development into long-lived plasma cells.

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Jul 3, 2016 | Posted by in RHEUMATOLOGY | Comments Off on B Cells
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