Allograft Preparation for ACL Reconstruction
William H. Warden III
INDICATIONS/CONTRAINDICATIONS
Allograft is an attractive option for anterior cruciate ligament (ACL) reconstruction, as there is no donor site morbidity and therefore less discomfort in the early postoperative period. In my experience, patients with allograft ACL reconstructions are clearly more comfortable immediately after surgery than patients who have received autograft. The most striking example I can recall is a patient who walked into the office, did a deep knee bend, and jumped into the air 1 week after a bilateral ACL reconstruction with no formal physical therapy.
One of the few strict contraindications to allograft reconstruction is a patient who is unwilling to accept the risk of disease transmission. Although there are few strict indications or contraindications, there are a number of advantages and disadvantages to consider.
Advantages
No donor site morbidity, therefore useful for:
Revision
Multiple ligament injuries
Early return to light work
Decreased anesthesia, operating room, tourniquet time
Cosmesis
Disadvantages
Risk of disease transmission
Cost
Patellar tendon, $1,800
Achilles tendon, $1,200
Soft tissue, $800
Offset by decreased operating room time
Potential for delayed incorporation
Although not entirely scientific, my personal bias is to avoid allograft in children and women of childbearing age, and lean toward allograft in patients who are age 50 or older.
PREOPERATIVE PLANNING
An early step involves a discussion with the patient of the risk of viral transmission.
Use tissue from a well-established tissue bank certified by the American Association of Tissue Banks (AATB) from donors under age 40. Ideally, grafts are obtained with sterile harvest, antibiotic soaks, low-dose radiation, and freezing. Higher dose irradiation in a radioprotectant solution is an emerging technique.
There are a number of allograft options including bone-patellar tendon-bone (BTB), Achilles tendon, quadriceps tendon, hamstring, and tibialis anterior. The choice is mainly surgeon preference. BTB is an excellent choice for a surgeon who prefers bone fixation, but this allograft can potentially carry more antigens. Preoperative planning is critical for BTB, to avoid graft-tunnel mismatch. This is not an issue with a large patient; for a small patient, request collagen lengths of 45 mm or less. If you cannot obtain a short graft, consider a soft tissue or single bone plug graft.
Always have a back-up graft. Occasionally, an allograft will appear discolored or macerated. A degenerative Achilles tendon may not be evident until the graft is sectioned. In case of the dreaded drop, one can potentially scrub an autograft, but it’s difficult to justify scrubbing an allograft that can simply be replaced by a backup.
SURGERY
Patient Positioning
Position the graft preparation table so that it does not interfere with entry into the room, draping, or setup of arthroscopy equipment. Ideally, you should have a good view of the arthroscopy monitor.
Technique
If it is certain that a graft will be required, graft preparation can begin before the patient is in the room. Thaw time ranges from 5 to 30 minutes including a soak in saline with “double antibiotic” (bacitracin and polymyxin B) solution. Grafts prepared with radioprotectant typically require three separate soaks over a total of 30 minutes. Inspect the graft for discoloration or signs of structural compromise. Trim excess fatty or loose connective tissue from the graft.
Achilles Tendon
Achilles tendon grafts have two unique features to address during graft preparation: the collagen insertion into the bone plug is angular and the tendinous portion is fan shaped. Although Achilles tendon grafts come with ample bone blocks, the nature of the tendinous insertion is such that the bone plug length is always short. Therefore, bone should not be removed from the distal end of the bone block unless necessary during final preparation (Fig. 17-1). A prominent pump bump at the posterior aspect of the plug may need to be trimmed along with a small amount of tendinous tissue (Fig. 17-2). The bone plug should be fashioned to fit easily through the sizing tube. We typically use a 10-mm bone plug. Next, shorten the graft to a length of approximately 120 mm and trim excess collagen from the sides (Fig. 17-3A). A No. 2 nylon passing suture is placed through the bone plug. Next, suture the distal portion of the graft by placing a No. 2 Ethibond suture 40 to 50 mm distal to the bone block. Distal interference screws seem to get better purchase on the graft if they are placed adjacent to a sutured graft; suturing can begin more distally if interference fixation is not used. The first suture placed should be used to tubularize the graft (see Fig. 17-3B). A second arm of the suture is run down opposite this and then a second suture is placed rotated 90 degrees to the initial suture. With each strand of the suture place one or two locked passes and then run distally.
Soft Tissue
Although soft tissue allows a tighter fit in the sizing tubes compared with bone plugs, avoid the tendency to make a collagen graft that is too large. A 10-mm bundle of collagen may not fit well in the knee of a petite gymnast.