Adrenal Cortex



Adrenal Cortex


Patricia A. Donohoue



NORMAL ADRENAL DEVELOPMENT AND STEROID HORMONE SYNTHESIS

The fetal adrenal cortex develops from coelomic mesothelium in proximity to the developing bipotential gonads. The cortex then separates from the gonads, which eventually migrate to their adult positions. For this reason, rests of adrenocortical tissue may appear along the paths of migration or near or within the gonads in adults. By the sixth week of gestation, steroid-producing cells appear in the adrenal cortex, and by the tenth week, the fetal zone (comprising 80% of the total volume) and the adult (definitive) zone are producing steroid hormones
under the stimulatory control of adrenocorticotropic hormone (ACTH) stimulation. The fetal zone, whose major products are estrogen and androgen precursors, begins to degenerate by the eighth month of gestation. At that time, the adult zone begins to develop and then differentiate into a mature adult cortex that secretes the three families of steroid hormones: mineralocorticoids, glucocorticoids, and androgens. This differentiation is not completed until the child is approximately 3 years of age.

The adult adrenal cortex constitutes approximately 90% of the mature gland and is composed of three zones. The outermost zone, the zona glomerulosa, accounts for 15% of the cortical volume and is the site of mineralocorticoid synthesis. The zona fasciculata constitutes 75% of the cortex, and the reticularis (the innermost zone) constitutes 10%. The zona fasciculata and zona reticularis are one functional unit, involved in glucocorticoid and androgen biosynthesis. The zona reticularis is thought to secrete steroids under basal conditions, and the zona fasciculata stores lipids for stress steroidogenesis.

All three groups of steroid hormones are produced from cholesterol, which is supplied by the circulation or produced endogenously. Under the stimulation of the anterior pituitary hormone ACTH, cholesterol is converted to pregnenolone. This conversion is the rate-limiting step in steroid hormone biosynthesis. Pregnenolone serves as the precursor for all three families of adrenal steroid hormones (Fig. 381.1).

Pituitary ACTH release is controlled by the hypothalamic peptide, corticotropin-releasing hormone (CRH). ACTH secretion also may be under the influence of the immune system. Normal ACTH secretion occurs in a diurnal pattern, which results in the normal diurnal fluctuation in serum cortisol levels: highest in the early morning and lowest in the evening. The serum cortisol level completes the feedback loop by stimulating (low cortisol levels) or suppressing (high cortisol levels)
hypothalamic CRH secretion and pituitary ACTH secretion. Serotonin stimulates and norepinephrine inhibits hypothalamic CRH secretion. The daily cortisol secretion rate initially was determined to be 12.1 ± 3 mg/m2 of body surface area (the total daily cortisol production increasing with growth), and this value still is widely accepted. More recent studies, employing different techniques, suggest a lower but more variable secretion rate. Based on the average daily production of cortisol and the potencies of various pharmacologic glucocorticoid preparations, recommendations can be made about the average daily dose of each that would be required for physiologic replacement (Table 381.1). Many preparations also have a mineralocorticoid effect (Table 381.2). In times of physiologic stress (fever or other illnesses), the recommendation is that the oral cortisol replacement doses be increased by threefold.






FIGURE 381.1. Adrenal steroid biosynthetic pathways. The 20-hydroxylase, 22-hydroxylase, and 20,22-desmolase are activities of the same CYP11A enzyme. Both 17-alpha-hydroxylase and 17,20-desmolase activities are properties of the same CYP17 enzyme. However, 11-beta-hydroxylase and CMO activities are properties of two different isozymes, CYP11B1 and CYP11B2, encoded by different adjacent genes. The single enzyme that has CMOI and CMOII activities is termed aldosterone synthase (CYP11B2). In addition, several isoforms of 3-beta-HSD exist, some of which are expressed in extraadrenal sites. 3β-HSD, 3-betahydroxysteroid dehydrogenase, Δ54-isomerase; CMO, corticosterone methyl oxidase; CYP11B1, 11-beta-hydroxylase; CYP11B2, 18-hydroxylase (CMOI) and 18-dehydrogenase (oxidase) (CMOII); CYP17, 17-alpha-hydroxylase and 17,20-desmolase (lyase); CYP21, 21-hydroxylase; CYP11A, 20-hydroxylase, 22-hydroxylase, 20,22-desmolase. (From Donohoue PA, Parker KL, Migeon CJ. Congenital adrenal hyperplasia. In: Scriver CR, Beaudet AL, Sly WS, Valle D, eds. The metabolic and molecular bases of inherited disease, 8th ed. New York: McGraw-Hill, 2001:4077, with permission from McGraw-Hill Publishers.)








TABLE 381.1. GLUCOCORTICOID DOSAGES
















Preparation Physiologic Replacement Dose*
Hydrocortisone 12.5 mg/m2/day IM or IV
15–25.0 mg/m2/day PO
Cortisone acetate 16 mg/m2/day IM or IV
25–32 mg/m2/day PO
Prednisolone 3–5 mg/m2/day PO
IM, intramuscularly; IV, intravenously; PO, per os (by mouth).
*Recommended doses for daily physiologic replacement of glucocorticoid. The actual optimal dose must be titrated for each patient.

Most cortisol circulates in the blood bound to cortisol-binding globulin (CBG), also known as transcortin, an alpha-globulin secreted by the liver. The free fraction of cortisol is the biologically active hormone. Estrogens increase levels of CBG, and liver disease and the nephrotic syndrome are associated with decreased levels of CBG. However, levels of free cortisol are unaffected by these conditions.

The major physiologic metabolic effects of cortisol are glycogen synthesis, gluconeogenesis, fat catabolism, and protein catabolism. At high levels, glucocorticoids induce a wide variety of metabolic changes, including immunosuppression, osteoporosis, glucose intolerance, increased gastric acid secretion, and altered central nervous system (CNS) function, resulting in psychiatric symptoms.

Mineralocorticoid (e.g., aldosterone) secretion is controlled mainly by the renin-angiotensin system and also by serum potassium levels. The cells of the zona glomerulosa, which have specific membrane receptors for angiotensin II, secrete aldosterone and its precursors. Stimulation of the adrenal cortex with ACTH produces only a transient increase in levels of aldosterone. The average daily aldosterone secretion rate is not related to body surface area and is similar for infants and adults (approximately 100 μg/day). The major physiologic effect of mineralocorticoids is exerted at the level of the distal convoluted tubule, where they promote retention of sodium and excretion of potassium.








TABLE 381.2. RELATIVE POTENCIES OF CORTICOSTEROIDS






































Steroid Preparation Effect*
Glucocorticoid Mineralocorticoid (mg)
Cortisone 100 100
Hydrocortisone 80 80
Prednisone 20 100
Prednisolone 20 100
Methylprednisolone 16 No effect
9-alpha-Fluorocortisol 5 0.2
Dexamethasone 2 No effect
*Relative glucocorticoid and mineralocorticoid potencies. The doses given for each preparation represent approximately equivalent clinical effects. For example, the mineralocorticoid effect of 0.2 mg 9-alphafluorocortisol (Florinef) is equivalent to the mineralocorticoid effect of 100 mg cortisone. Prednisone and prednisolone are potent glucocorticoids and weak mineralocorticoids.


The factors that control secretion of adrenal androgen are not understood as well. In prepubertal children, the production of adrenal androgens is very low. At puberty, their production increases and normal adrenarche occurs. The most potent adrenal androgen is androstenedione, which is converted outside the adrenal gland to the more potent androgen testosterone. At very high levels, dehydroepiandrosterone, a weak androgen, may exert androgenic effects. In pubertal and adult males, the adrenal gland contributes little to the total production of androgen. However, in pubertal and adult females, at least 50% of the circulating testosterone is derived from adrenal androstenedione.


TESTS OF ADRENOCORTICAL FUNCTION


Static Tests

The static tests of adrenocortical function provide important but limited information and often must be accompanied by dynamic testing in the diagnostic evaluation of adrenal disorders. Normal values for some levels of adrenal steroids and responses to dynamic tests are given in Table 381.3.

The level of serum cortisol is measured by immunoassay and is interpretable only if the time of day that the sample was obtained is known. If the cortisol level is subnormal at 8 AM, the time of a normal peak, hypocortisolism is suspected. However, this test does not discriminate among primary adrenal failure, ACTH deficiency, or an enzymatic defect in the biosynthesis of cortisol. A low level of cortisol late in the day is normal and has little value in the assessment of adrenal failure. However, if the level is elevated in a nonstressed patient, it may indicate absence of the normal pattern of diurnal variation often seen in Cushing syndrome. Because the level of serum cortisol rises briskly in response to such stresses as fever, trauma, surgery, fear, or anxiety, single determinations of levels of cortisol cannot be used reliably to diagnose hypercortisolism.

Determination of the level of serum ACTH is useful only if it is accompanied by other tests of adrenal function. The diurnal variation in levels of cortisol is preceded by similar fluctuations in levels of ACTH. An extremely elevated level of ACTH in the setting of subnormal levels of serum cortisol suggests primary adrenal failure. However, because ACTH mediates the rise in serum cortisol in response to stress, its levels vary widely.

Mineralocorticoid status is assessed by measuring concentrations of serum electrolytes and plasma renin activity (PRA), which is elevated in mineralocorticoid deficiency. PRA is a measure of the rate of conversion of angiotensinogen to angiotensin I. Such factors as blood pressure, posture, intake of sodium, and renal function affect PRA and must be considered in the interpretation of the test result. The PRA assay may be useful in monitoring the adequacy of mineralocorticoid replacement therapy. A direct renin assay also is now available. Levels of plasma or urinary excretion of aldosterone and deoxycorticosterone also are useful for assessing secretion of mineralocorticoids.








TABLE 381.3. NORMAL PLASMA AND URINARY STEROID HORMONE LEVELS WITH STATIC AND DYNAMIC TESTS



































































































































































Test Valuesa
Static tests
Plasma cortisolb   μg/dL  
Premature infants (day 4)
   26–28 weeks 1–11 (6.0)
   31–35 weeks 2.5–9.1 (6.4)
Full-term infants
   3 days 1.7–14 (6.2)
   7 days 2–11 (4.4)
   31 days–11 months 2.8–23 (9.4)
Children
   12 months–15 years (0800) 3–21 (9.8)
Adults
   0800 8–19 (11.0)
   1600 4–11 (5.9)
mg/g creatinine mg/24 hours
Urinary 17-hydroxy corticosteroids (17-OHCS) Prepubertal children
1–4 years 1.7–6.4 (4.1) 0.2–2.5 (0.8)
5–9 years 2.2–6.0 (3.5) 0.5–2.5 (1.2)
Pubertal children and adults
   Male 2.4–4.3 (3.2) 3–10 (6.4)
   Female 1.6–3.6 (2.3) 2–6 (2.8)
  μg/g creatinine μg/24 hours
Urinary free cortisol Prepubertal children 7–25 (15) 3–9 (5.2)
Adult men 7–45 (21) 11–84 (40)
Adult women 9–32 (19) 10–34 (20)
Pregnancy 14–59 (38) 16–60 (47)
  (μg/dL)  
Salivary cortisol Prepubertal children
0800 0.17–1.2  
1600 0.10–0.33
2300 0.03–0.19
Adults
0800 0.18–0.95
1600 0.10–0.28
2300 0.05–0.17
Postdexamethasone (after 1 mg @ 2300)
0800 <0.1
Dynamic tests
Adrenal capacity Plasma cortisol at 1 hour is double baseline level and >18 μg/dL
   Rapid IV test: 0.25 mg Cortrosyn over 1 minute:
   Prolonged test: 20 U/m2 Acthar gel IM every 12 hours for 3 days, or 0.25 mg Cortrosyn IV over 8–12 hours beginning at 0800 for 3 days: Urinary 17-OHCS: threeto fivefold increase over baseline
ACTH capacityc
   Oral metyrapone 15 mg/kg every 4 hours six times from 0800 to 1600: Urinary 17-OHCS increase twoto fourfold or serum 11-deoxy-cortisol increase to >10 ng/dL
   Oral metyrapone: 30–40 mg/kg (up to 3.0 g) at midnight: 8 AM: Increase in serum 11-deoxycortisol to >7 μg/dL, with a decrease in serum cortisol to <5 μg/dL
   Regular insulin 0.05–0.10 U/kg IV or glucagon 0.1 mg/kg IM: Rise in serum cortisol by 10 μg/dL or to >20 μg/dL
Pituitary suppression tests:
Overnight test
20 μg/kg dexamethasone PO (up to 1.0 mg*) at 2300, with serum cortisol level at 0800 the following morning. Normal response: 0800 cortisol <5 μg/dl rules against Cushing syndrome,
Low-dose test
48 hour, 2 mg/day (0.5 mg PO q6h) Normal response: 0800 cortisol level obtained 2 hours after the last dose of dexamethasone <1.8 μg/dL rules against Cushing syndrome§
48 hour, 2 mg/day test combined with CRH stimulation test at 0800, 2 hours after the last dose of dexamethasone Normal response: serum cortisol <1.4 μg/dl, 15 minutes after CRH 1 μg/kg IV (or 100 μg IV in adults) rules against Cushing syndrome
High-dose test for differential diagnosis of ACTH-dependent Cushing syndrome||
48 hour, 8 mg/day (2 mg dexamethasone PO q6h) Suppression of urinary 17-OHCS by >50% consistent with pituitary source of ACTH
Overnight test (8 mg dexamethasone PO at 2300) Serum cortisol at 0800 the mornings before and after show suppression of serum cortisol by >50% suggests pituitary source of ACTH
ACTH, adrenocorticotropic hormone; CRH, corticotropin-releasing hormone; IM, intramuscularly; IV, intravenously.
*The use of a 1.5or 2.0-mg dose offers no better discrimination between the presence and absence of Cushing syndrome.
†There are some patients with Cushing syndrome whose serum cortisol will suppress to <5 μg/dL. A cutoff of ≤1.8 μg/dL effectively excludes all cases of Cushing syndrome.
‡Some suggest simultaneous measurement of a dexamethasone level to ensure compliance if the testing is performed as an outpatient.
§The original report of Liddle (Tests of pituitary-adrenal suppressibility in the diagnosis of Cushing syndrome. J Clin Endocrinol Metab 1960;20:1539) described suppression of urinary 17-hydroxycorticosteroid excretion as the expected outcome.
||This test is utilized after excess ACTH has been demonstrated. It has been largely replaced by high-resolution radiologic studies, bilateral inferior petrosal sampling, and CRH stimulation testing.
aNormal ranges and means (in parentheses) for static test values are based on reference ranges from Esoterix Laboratories, Calabasas Hills, California, and are used with permission.
bStress or anxiety may cause elevation of cortisol levels far above the stated normal range.
cIn many centers, metyrapone no longer is available for diagnostic testing. Insulin-induced hypoglycemia or glucagon stimulation will be the tests of choice for ACTH capacity. The specific details of the glucagon stimulation test are described in Vanderschueren-Lodeweyckx M, Wolter R, Malvaux P, et al. The glucagon stimulation test: effect on plasma growth hormone and on immunoreactive insulin, cortisol, and glucose in children. J Pediatr 1974;85:182.

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Jul 24, 2016 | Posted by in ORTHOPEDIC | Comments Off on Adrenal Cortex

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