Anatomy and Neurophysiology




The electromyographer need not have detailed knowledge of all the electrical and chemical events that occur at a molecular level in order to perform an electrodiagnostic (EDX) study. However, every electromyographer must have a basic understanding of anatomy and physiology in order to plan, perform, and properly interpret an EDX study. In the everyday evaluation of patients with neuromuscular disorders, nerve conduction studies (NCSs) and electromyography (EMG) serve primarily as extensions of the clinical examination. Knowledge of gross nerve and muscle anatomy is required to be able to perform these studies. For NCSs, one needs to know the location of the various peripheral nerves and muscles so that the stimulating and recording electrodes are properly positioned. For the needle EMG study, knowledge of gross muscle anatomy is crucial for inserting the needle electrode correctly into the muscle being sampled. On the microscopic level, knowledge of nerve and muscle anatomy and basic neurophysiology are required to appreciate and interpret the EDX findings both in normal individuals and in patients with various neuromuscular disorders. Lastly, knowledge of anatomy and physiology are crucial to understanding the technical aspects of the EDX study and appreciating its limitations and potential pitfalls.


Anatomy


The strict definition of the peripheral nervous system includes that part of the nervous system in which the Schwann cell is the major supporting cell, as opposed to the central nervous system in which the glial cells are the major support cells. The peripheral nervous system includes the nerve roots, peripheral nerves, primary sensory neurons, neuromuscular junctions (NMJs), and muscles ( Figure 2–1 ). Although not technically part of the peripheral nervous system, the primary motor neurons (i.e., anterior horn cells), which are located in the spinal cord, are often included as part of the peripheral nervous system as well. In addition, cranial nerves III through XII are also considered to be part of the peripheral nervous system, being essentially the same as peripheral nerves, except that their primary motor neurons are located in the brainstem rather than the spinal cord.




FIGURE 2–1


Elements of the peripheral nervous system.

The peripheral nervous system includes the peripheral motor and sensory nerves; their primary neurons, the anterior horn cells, and dorsal root ganglia; the neuromuscular junctions (NMJs); and muscle. The dorsal root ganglion, a bipolar cell located distal to the sensory root, is anatomically different from the anterior horn cell. Consequently, lesions of the nerve roots result in abnormalities of motor nerve conduction studies but do not affect the sensory conduction studies, as the dorsal root ganglion and its peripheral nerve remain intact.


The primary motor neurons, the anterior horn cells , are located in the ventral gray matter of the spinal cord. The axons of these cells ultimately become the motor fibers in peripheral nerves. Their projections first run through the white matter of the anterior spinal cord before exiting ventrally as the motor roots . In contrast to the anterior horn cell, the primary sensory neuron, also known as the dorsal root ganglion (DRG), is not found within the substance of the spinal cord itself but rather lies outside the spinal cord, near the intervertebral foramen. The dorsal root ganglia are bipolar cells with two separate axonal projections. Their central projections form the sensory nerve roots . The sensory roots enter the spinal cord on the dorsal side to either ascend in the posterior columns or synapse with sensory neurons in the dorsal horn. The peripheral projections of the DRGs ultimately become the sensory fibers in peripheral nerves. Because the DRGs lie outside the spinal cord, this results in a different pattern of sensory nerve conduction abnormalities, depending on whether the lesion is in the peripheral nerve or proximal to the DRG, at the root level (see Chapter 3 ).


Motor and sensory roots at each spinal level unite distal to the DRG to become a mixed spinal nerve . There are 31 pairs of spinal nerves (8 cervical, 12 thoracic, 5 lumbar, 5 sacral, 1 coccygeal; Figure 2–2 ). Each spinal nerve divides into a dorsal and ventral ramus ( Figure 2–3 ). Unlike the dorsal and ventral nerve roots, the dorsal and ventral rami both contain motor and sensory fibers. The dorsal ramus runs posteriorly to supply sensory innervation to the skin over the spine and muscular innervation to the paraspinal muscles at that segment. The ventral ramus differs, depending on the segment within the body. In the thoracic region, each ventral ramus continues as an intercostal nerve . In the lower cervical to upper thoracic (C5–T1) region, the ventral rami unite to form the brachial plexus ( Figure 2–4 ). In the mid-lumbar to sacral regions, the ventral rami intermix to form the lumbosacral plexus ( Figure 2–5 ).




FIGURE 2–2


Spinal cord and nerve roots.

The spinal cord is divided into 31 segments (8 cervical, 12 thoracic, 5 lumbar, 5 sacral, and 1 coccygeal). At each segment, motor and sensory fibers leave the spinal cord as nerve roots before exiting the bony spinal column. In the adult, the spinal cord usually ends at the level of the L1 vertebra. Consequently, below this level, only the lumbosacral nerve roots, known as the cauda equina, are present within the spinal column.

(From Haymaker, W., Woodhall, B., 1953. Peripheral nerve injuries. WB Saunders, Philadelphia, with permission.)



FIGURE 2–3


Nerve roots and rami.

The motor root, originating from anterior horn cells, leaves the cord ventrally, whereas the sensory root enters the cord on the dorsal side. Immediately distal to the dorsal root ganglion, the motor and sensory roots come together to form the spinal nerve. Each spinal nerve quickly divides into a dorsal and ventral ramus. Each ramus contains both motor and sensory fibers. The dorsal rami supply sensation to the skin over the spine and muscular innervation to the paraspinal muscles. The ventral rami continue as intercostal nerves in the thoracic region. In the lower cervical region, the ventral rami fuse to form the brachial plexus. In the mid-lumbar through sacral segments, the ventral rami intermix to form the lumbosacral plexus.



FIGURE 2–4


Brachial plexus.

The ventral rami of the C5–T1 nerve roots intermix to form the brachial plexus between the neck and shoulder. From the brachial plexus, the major upper extremity peripheral nerves are derived.

(From Hollinshead, W.H., 1969. Anatomy for surgeons, volume 2: the back and limbs. Harper & Row, New York, with permission.)



FIGURE 2–5


Lumbosacral plexus.

The L1–S4 nerve roots intermix in the pelvis to form the lumbosacral plexus. From this plexus, the individual major peripheral nerves of the lower extremity are derived.

(From Mayo Clinic and Mayo Foundation. 1956. Clinical examinations in neurology. WB Saunders, Philadelphia, with permission.)


Within each plexus, motor and sensory fibers from different nerve roots intermix to ultimately form individual peripheral nerves . Each peripheral nerve generally supplies muscular innervation to several muscles and cutaneous sensation to a specific area of skin, as well as sensory innervation to underlying deep structures. Because of this arrangement, motor fibers from the same nerve root supply muscles innervated by different peripheral nerves, and sensory fibers from the same nerve root supply cutaneous sensation in the distribution of different peripheral nerves. For instance, the C5 motor root supplies the biceps (musculocutaneous nerve), deltoid (axillary nerve), and brachioradialis (radial nerve), among other muscles ( Figure 2–6 ). Similarly, C5 sensory fibers innervate the lateral arm (axillary nerve) and forearm (lateral antebrachial cutaneous sensory nerve), in addition to other nerves.




FIGURE 2–6


Myotomal and peripheral nerve innervation.

Motor fibers from one nerve root, a myotome, supply muscles innervated by different peripheral nerves. For example, the C5 motor root supplies the biceps (musculocutaneous nerve), deltoid (axillary nerve), and brachioradialis (radial nerve), among other muscles.

(Adapted from Haymaker, W., Woodhall, B., 1953. Peripheral nerve injuries. WB Saunders, Philadelphia, with permission.)


All muscles supplied by one spinal segment (i.e., one nerve root) are known as a myotome , whereas all cutaneous areas supplied by a single spinal segment are known as a dermatome ( Figure 2–7 ). For both myotomes and dermatomes, there is considerable overlap between adjacent segments. Because of the high degree of overlap between spinal segments, a single root lesion seldom results in significant sensory loss and never in anesthesia. Likewise, on the motor side, even a severe single nerve root lesion usually results in only mild or moderate weakness and never in paralysis. For instance, a severe lesion of the C6 motor root causes weakness of the biceps; however, paralysis would not occur because C5 motor fibers also innervate the biceps. In contrast, a severe peripheral nerve lesion usually results in marked sensory and motor deficits because contributions from several myotomes and dermatomes are affected.






FIGURE 2–7


Dermatomes.

The cutaneous area supplied from one spinal segment (i.e., one sensory nerve root) is known as a dermatome. Despite the apparent simplicity of dermatomal charts, in actuality, there is a wide overlap of adjacent dermatomes. Consequently, a nerve root lesion, even if severe, never results in anesthesia but rather altered or decreased sensation.

(From O’Brien M.D., 1986. Aids to the examination of the peripheral nervous system. Baillière Tindall, London.)


At the microscopic level, nerve fibers are protected by three different layers of connective tissue: the epineurium, perineurium, and endoneurium ( Figure 2–8 ). The thick epineurium surrounds the entire nerve and is in continuity with the dura mater at the spinal cord level. Within the epineurium, axons are grouped into fascicles, surrounded by perineurium . A final layer of connective tissue, the endoneurium , is present between individual axons. Effectively, a blood–nerve barrier is formed by the combination of vascular endothelium supplying the nerve and the connective tissue of the perineurium. Together, the three layers of connective tissue give peripheral nerve considerable tensile strength, usually in the range of 20 to 30 kg. However, the weakest point of a nerve occurs where the nerve roots meet the spinal cord, where the nerve can sustain only 2 to 3 kg of force. For this reason, nerve root avulsion may occur after a significant trauma and especially after a stretch injury.




FIGURE 2–8


Internal peripheral nerve anatomy.

Myelinated fibers are recognized as small dark rings (myelin) with a central clearing (axon) in this one micron thick, semi-thin section of plastic embedded nerve tissue. The endoneurium is present between axons. Axons are grouped into fascicles, surrounded by perineurium (small arrows). Surrounding the entire nerve is the last layer of connective tissue, the epineurium (large arrow).




Physiology


The primary role of nerve is to transmit information reliably from the anterior horn cells to muscles for the motor system and from the sensory receptors to the spinal cord for the sensory system. Although functionally nerves may seem similar to electrical wires, there are vast differences between the two. At the molecular level, a complex set of chemical and electrical events allows nerve to propagate an electrical signal.


The axonal membrane of every nerve is electrically active. This property results from a combination of a specialized membrane and the sodium/potassium (Na + /K + ) pump ( Figure 2–9 ). The specialized axonal membrane is semipermeable to electrically charged molecules (anions and cations). The membrane is always impermeable to large negatively charged anions, and it is relatively impermeable to sodium in the resting state. This semipermeable membrane, in conjunction with an active Na + /K + pump that moves sodium outside in exchange for potassium, leads to concentration gradients across the membrane. The concentration of sodium is larger outside the membrane, whereas the concentration of potassium and larger anions is greater inside. The combination of these electrical and chemical gradients results in forces that create a resting equilibrium potential. At the nerve cell soma, this resting membrane potential is approximately 70 mV negative inside compared with the outside; distally in the axon it is approximately 90 mV negative.




FIGURE 2–9


Resting membrane potential.

At rest, the axonal membrane is negatively polarized, inside compared to outside. This resting potential results from the combination of a membrane that is semipermeable to charged particles and an active Na + /K + pump. At rest, the concentration of Na + and Cl is higher in the extracellular space, with the concentration of K + and large anions (A ) greater inside the axon.

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Mar 1, 2019 | Posted by in PHYSICAL MEDICINE & REHABILITATION | Comments Off on Anatomy and Neurophysiology

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